General Cell Based Assays
Apexbio Technology LLC JC-10 Mitochondrial Membrane Potential Assay Kit(Synonyms: JC-10 Assay Kit, JC-10 MMP Assay Kit, JC-10 mitochondrial dye assay), 100T.
The JC-10 Mitochondrial Membrane Potential Assay Kit utilizes the fluorescent probe JC-10 to assess mitochondrial membrane potential ( m) a critical marker for mitochondrial function and early apoptosis detection JC-10 selectively accumulates in mitochondria with intact membrane potential forming red-fluorescent aggregates Upon loss of m associated with apoptosis or mitochondrial dysfunction JC-10 disperses into monomeric forms emitting green fluorescence The mitochondrial membrane potential is therefore indicated by changes in the ratio between red and green fluorescence intensity This assay is suitable for studies involving cultured cells isolated mitochondria or tissue samples and is commonly employed in apoptosis research and mitochondrial functional analysis
Aat Bioquest iFluor™ 350 succinimidyl ester
AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins.
Aat Bioquest iFluor™ 647 azide
AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins.
Aat Bioquest Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*
The detection of intracellular hydroxyl radical is of central importance to understanding proper cellular redox regulation and the impact of its dysregulation on various pathologies.
ABclonal Technology 12 Lipoxygenase/ALOX12
This gene encodes a member of the lipoxygenase family of proteins The encoded enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins The encoded enzyme and its reaction products have been shown to regulate platelet function Elevated expression of this gene has been observed in pancreatic islets derived from human diabetes patients Allelic variants in this gene may be associated with susceptibility to toxoplasmosis Multiple pseudogenes of this gene have been identified in the human genome
Eurofins Discoverx BEVACIZUMAB BIOASSAY HS, 2-PLA
PathHunter Bevacizumab Bioassay HS Kit, 2-Plate. (93-0996Y1-00165).
Eurofins Discoverx cAMP Hunter eXpress CCR6 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0069E2CP2L)
The cAMP Hunter CCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL20) based activation of CCR6 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CCR6, a naturally Gi/Go coupled GPCR. Upon activation of CCR6, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CCR6 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0069E2CP2M)
The cAMP Hunter CCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL20) based activation of CCR6 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CCR6, a naturally Gi/Go coupled GPCR. Upon activation of CCR6, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress cyno CXCR2 HEK 293 β-Arrestin GPCR Assay, 2pl Kit. (93-0981E1CCP0M)
The PathHunter cyno CXCR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of cyno CXCR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno CXCR2 and EA-tagged β-Arrestin-2. Activation of cyno CXCR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR35 Activated GPCR Internalization Assay, 10pl Kit. (93-0968E3CP5L)
The PathHunter GPR35 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GPR35 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mGCGR CHO-K1 β-Arrestin-1 GPCR Assay, 2-Plate Kit. (93-0973E2MCP2M)
The PathHunter mGCGR (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. glucagon) based activation of mGCGR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGCGR and EA-tagged β-Arrestin-1. Activation of mGCGR stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR35 Activated GPCR Internalization Assay, 2pl Kit. (93-0968E3CP5M)
The PathHunter GPR35 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GPR35 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems QuantiChrom™ Detergent Assay Kit. For quantitative determination of detergents in e.g. water and soil samples.
For quantitative determination of detergents in e.g. water and soil samples. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM. Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Method: 560nm and 650nm. Samples: Biological and environmental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.